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課程名稱 |
小分子核酸與基因沉默機制
ADVANCED SMALL RNA AND MECHANISM OF GENE SILENCING |
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開課學期 |
99-1 |
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授課對象 |
生物資源暨農學院 生物科技研究所 |
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授課教師 |
林詩舜 |
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課號 |
Biot8016 |
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課程識別碼 |
642ED0180 |
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班次 |
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學分 |
3 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期二2,3,4(9:10~12:10) |
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上課地點 |
生技所410 |
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備註 |
本課程以英語授課。本課程以英語授課。
限博士班
總人數上限：20人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
Course description: This half-year graduate course aims to introduce basic gene silencing concepts and research techniques available to address gene silencing questions in plant as well as other organisms. Students are expected to develop the ability to critically evaluate and interpret published research articles. They will also learn to extract information from international conferences by listening to experts of each field introducing their research.
Outline:
1. Introduction - overview
2. Sense co-suppression in plant
3. Transgene co-suppression in animals
4. Plant virus resistance vs. co-suppression
5. Transcriptional gene silencing (TGS)
6. Post-transcriptional gene silencing (PTGS)
7. Virus-induce gene silencing (VIGS)
8. Silencing signal transportation
9. Small RNA
10. siRNA pathway
11. miRNA pathway
12. Function of DICER
13. Function of RNA-induce silencing complex (RISC)
14. Functions of DICER-like proteins (DCLs) in plant
15. DCL1 vs. miRNA biogenesis
16. Function of DCL2 and nat-siRNA
17. Function of DCL3 and DNA methylation
18. Function of DCL4 and trans-siRNA
19. Argonature family
20. Suppression of gene silencing
21. RNAi technology
22. miRNA technology |
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課程目標 |
本課程將針對動物與植物之最近基因沉默機制之最新發展進行討論，著重在技術之演進與學理之闡釋。學習者將具備研讀及評估當代基因沉默機制相關文獻之能力。 |
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課程要求 |
具備分子生物學、生物化學之基礎知識 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
本課程主題相關之近期英文期刊文獻，期刊種類包括 Cell、Development、Dev Biol、Dev Genet、Genes Dev、EMBO J、J Biol Chem、J Cell Biol、J Mol Biol、Mol Cell Biol、Nature、The Plant Cell、Plant Mol Biol、Plant J、Plant Physiol、Proc Natl Acad Sci USA、Science 等。 |
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評量方式
(僅供參考) |
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